Simple simultaneous detection of three pathogenic bacterial species in cyclamen using the Multiplex PCR method
نویسندگان
چکیده
منابع مشابه
Simultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method
In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...
متن کاملdevelopment of multiplex pcr for simultaneous detection of three pathogenic shigella species.
shigella species are among the common causes of bacterial diarrhoeal diseases. traditional detection methods are time-consuming resulting in delay in treatment and control of shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of shigella spp. in this study a rapid multiplex pcr were developed for simultaneous detection of three pathogenic ...
متن کاملDevelopment of Multiplex PCR for Simultaneous Detection of Three Pathogenic Shigella Species
BACKGROUND Shigella species are among the common causes of bacterial diarrhoeal diseases. Traditional detection methods are time-consuming resulting in delay in treatment and control of Shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of Shigella spp. In this study a rapid multiplex PCR were developed for simultaneous detection of three ...
متن کاملsimultaneous detection of helicobacter genus and helicobacter pylori species using a multiplex pcr method
in order to improve simultaneous detection and identification of helicobacter genus in general and helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex pcr. in this study, two pairs of primers: hcom1 and hcom2 specific for helicobacter genus, hicd1 and hicd2 specific for helicobacter pylori species were used. to determine the sensitivity of...
متن کاملUse of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions.
A multiplex PCR procedure was developed for the simultaneous detection of Alloiococcus otitidis, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae in middle ear effusions (MEEs) from patients with chronic otitis media with effusion. The bacterial 16S rRNA gene was chosen as the target, and the procedure used one common lower primer and four species-specific upper prime...
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ژورنال
عنوان ژورنال: Annual Report of The Kansai Plant Protection Society
سال: 2012
ISSN: 0387-1002,1883-6291
DOI: 10.4165/kapps.54.111